Shifting Limitation

While not common in an industrial bioprocess, there are systems in which growth rate can be limited by more than one nutrient. For example, a waste treatment unit could have feed rich in carbohydrates and low in nitrogen and later be faced with feed low in carbohydrates and rich in nitrogen. The nutrient in lowest proportion would change, and there may be transitions during which more than one is below a concentration that would give  (maximum growth rate coefficient).

We can also envision a process operated intentionally at low concentration of nitrogenous nutrients to restrict growth and at low sugar concentration to favor a particular pathway.

There is a presentation that covers our attempts to develop simple equations for toxic and/or multiple growth-limiting nutrients. Use "BACK" repeatedly to return to here if you try it.

When confronted with a normal substrate such as glucose, most organisms will not waste energy for elaborating the enzymes for an uncommon substrate such as lactose. Only when the concentration of glucose falls to a low level will the enzymes for the other sugar be synthesized. There are actual data by Egli, et al., (1986).

There is a Java exercise that shows a likely situation in continuous culture for a transition from sugar limitation to oxygen limitation in continuous culture.

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