Precipitation by Metal Ions

In this type of precipitation, a metal ion will bind to a part of the protein. An advantage of this type of precipitation is that they have great precipitating power in a dilute solution. These ions can be classified into three groups. Ions such as Mn2+, Fe2+, Co2+, Ni2+, Zn2+, and Cd2+ will bind strongly to carboxylic acids and to nitrogenous compounds. Ca2+, Ba+2, Mg+2, and Pb+2 will mainly bind to carboxylic acids. Finally, Ag+, Hg2+, and Pb2+ will bind strongly to sulphydryl groups.

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